FastQCFastQC Report
Thu 2 Jun 2016
DRR028437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345648
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22040.16378726085870896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.054.1994441
TCAACGC19600.052.30474
ATCAACG19950.051.0115243
CAACGCA21500.048.0254635
GTATCAA21400.047.8942381
GTACATG40700.044.0244671
AACGCAG23250.043.7644776
TACATGG41000.040.6465262
TATCAAC28700.036.227312
ACGCAGA27200.035.7838637
CGCAGAG26300.035.056518
ACATGGG48350.032.1823733
GCAGAGT29700.026.8889229
GAGTACA30300.025.5522381
GTATAGA10000.025.0703931
CATGGGA21600.024.9259134
CATGGGG32000.024.8917684
AGTACAT32650.023.2623142
GTATAAT12550.022.3265761
GTATATA10950.018.8549841
Produced by FastQC (version 0.11.5)