FastQCFastQC Report
Thu 2 Jun 2016
DRR028437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345648
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.1461749283616518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17440.12960298681378787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.084.085071
GTATCAA37900.057.3469351
TCAACGC44200.048.648764
ATCAACG44250.048.5937923
CAACGCA44300.048.5389485
AACGCAG48350.044.3233766
TATCAAC50150.042.8768772
ACGCAGA55100.038.1051837
CGCAGAG54500.037.5947848
GTACATG41400.034.6491851
TACATGG40650.034.1962362
ACATGGG40550.033.0307543
GCAGAGT60450.032.69679
CAACGCG900.00365332232.1777084
GTGGTAT9150.026.1288931
CATGGGA21550.024.5252364
CAGAGTA59350.023.5436739
CATGGGG26650.023.0918834
TGGTATC9950.021.8290982
GAGTACA31250.021.5606121
Produced by FastQC (version 0.11.5)