FastQCFastQC Report
Thu 2 Jun 2016
DRR028436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632126
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26280.1610169803066675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.057.7581561
TCAACGC24350.050.584944
GTATCAA26500.049.2455181
ATCAACG25150.048.3786543
CAACGCA26550.046.1155435
AACGCAG27600.042.7482876
GTACATG46850.037.7694741
ACGCAGA29700.036.9945377
TACATGG46400.036.2347032
TATCAAC35000.036.0276452
CGCAGAG30350.034.683198
ACATGGG51600.030.725333
GCAGAGT37200.026.345969
CATGGGA25450.026.0830554
TAGACCG1500.001352243624.5856935
GAGTACA34900.022.3933831
ACGCAGT2603.1697564E-622.3370788
CATGGGG32700.022.104574
AGTACAT37100.019.2799382
TAACGCA2409.4452413E-418.439274
Produced by FastQC (version 0.11.5)