FastQCFastQC Report
Thu 2 Jun 2016
DRR028436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632126
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.18552489207328354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.16536713464524183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.083.5501561
GTATCAA49000.065.223021
TCAACGC56800.055.1931724
ATCAACG56950.055.0477943
CAACGCA58600.053.4978185
AACGCAG60850.051.2817086
TATCAAC65100.048.8235442
ACGCAGA68900.044.7647487
CGCAGAG70000.043.957878
GCAGAGT77150.038.19489
GTACATG49900.035.1457821
TACATGG51550.032.72452
ACATGGG49550.032.4380763
GGTTACG1450.00122973624.9896641
CATGGGA21350.024.7554454
CATGGGG31250.024.5584874
CAGAGTA77150.023.6488699
GTGGTAT11350.020.4320771
TGTACCG2308.0382515E-418.887275
AGAGTAC74000.017.83748810-14
Produced by FastQC (version 0.11.5)