FastQCFastQC Report
Thu 2 Jun 2016
DRR028435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076400
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16350.15189520624303232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12300.047.9398424
GTATCAA13200.047.4495541
ATCAACG12650.046.600083
GTACATG27500.044.2118231
CAACGCA13300.041.5683635
GGTATCA9250.040.6270831
TACATGG27150.038.8108672
AACGCAG14400.037.8738486
TATCAAC17850.033.8503232
ACATGGG31400.031.9151173
CGCAGAG16600.030.6202478
ACGCAGA16950.029.5671487
GTACGGA1450.001114488225.4139546
GAGTACA19350.025.1333351
GTAAACG1500.001359513424.5621223
CATGGGG23600.023.7363194
AGAGTAC16450.021.957535
AGTACAT20850.021.9115332
CATGGGA14450.021.4236374
GCAGAGT20050.020.6442769
Produced by FastQC (version 0.11.5)