FastQCFastQC Report
Thu 2 Jun 2016
DRR028435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076400
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.18970642883686362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.1686176142697882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.089.994971
GTATCAA32250.055.7135471
ATCAACG36750.049.054423
TCAACGC36550.048.9266744
CAACGCA38400.046.758075
AACGCAG40150.044.5397226
TATCAAC40850.043.953732
ACGCAGA44950.039.1392677
CGCAGAG45150.038.8055428
GTACATG29400.035.2393231
TACATGG30050.034.2121242
GCAGAGT50850.033.316639
ACATGGG30450.031.8605793
TGGTATC7500.030.8904932
GTGGTAT8850.027.8339271
GTTACGT1050.00773466127.6000291
GTATTAG8950.023.4754431
TCTGTCG3102.4541805E-823.354718
CATGGGG22200.023.1548254
CATGGGA15350.022.6396144
Produced by FastQC (version 0.11.5)