FastQCFastQC Report
Thu 2 Jun 2016
DRR028434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592242
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25250.15858142166831424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.055.3032841
GTATCAA24100.047.7306331
TCAACGC23950.044.657324
ATCAACG24300.044.3061833
CAACGCA26600.041.3189055
AACGCAG27700.037.805456
GTACATG44700.037.4462171
TATCAAC32150.034.403292
TACATGG45900.033.414912
ACGCAGA31750.031.1268377
ACATGGG49400.030.1538073
CGCAGAG31100.030.1162098
GAGTACA34600.025.1475431
TAGACCG1550.001635850523.7948255
CATGGGG36800.022.850054
GCAGAGT35300.022.6242969
AGTACAT37150.021.833512
CATGGGA20650.021.0747324
ATAGTAC11300.018.9250623
CAGAGTA35050.018.4356779
Produced by FastQC (version 0.11.5)