FastQCFastQC Report
Thu 2 Jun 2016
DRR028434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592242
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.13019377707660015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18360.11530910502298018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.082.421171
GTATCAA41100.060.4779621
ATCAACG46500.052.7799073
TCAACGC46450.052.680864
CAACGCA48250.050.865615
AACGCAG50200.049.4666256
TATCAAC53200.045.8606452
ACGCAGA58400.041.5292247
CGCAGAG59100.040.547348
GCAGAGT68450.033.3164679
GTACATG44400.031.5006281
TACATGG44950.028.5079152
ACATGGG45900.027.4446963
CAGAGTA64900.023.2028059
GTGGTAT10150.022.1330131
GAGTACA35350.021.5250781
ACTGTCG2053.7030675E-421.1894288
CATGGGG33150.020.7473184
TGGTATC10650.019.034012
CCTATAC9200.018.0993043
Produced by FastQC (version 0.11.5)