FastQCFastQC Report
Thu 2 Jun 2016
DRR028433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6502
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA430.6613349738541987No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA420.6459550907413103No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA340.5229160258382036No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC320.492156259612427No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT240.3691171947093202No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA230.3537373115964319No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.3383574284835435No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG210.32297754537065515No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA200.30759766225776686No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA200.30759766225776686No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC170.2614580129191018No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT150.23069824669332512No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC140.2153183635804368No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG130.19993848046754845No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT120.1845585973546601No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG120.1845585973546601No Hit
CGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAA120.1845585973546601No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC120.1845585973546601No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG110.16917871424177175No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG110.16917871424177175No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA110.16917871424177175No Hit
ACCGACGCTGACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCG110.16917871424177175No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC100.15379883112888343No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC100.15379883112888343No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG100.15379883112888343No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA100.15379883112888343No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA90.13841894801599508No Hit
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG90.13841894801599508No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT90.13841894801599508No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT80.12303906490310675No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA80.12303906490310675No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT80.12303906490310675No Hit
ATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCATCACCA80.12303906490310675No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT70.1076591817902184No Hit
CGTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAA70.1076591817902184No Hit
AATCACCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGC70.1076591817902184No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC70.1076591817902184No Hit
CATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTC70.1076591817902184No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT70.1076591817902184No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT70.1076591817902184No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC70.1076591817902184No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT70.1076591817902184No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGG100.006447917148.507931
TCGTGGA100.006447917148.507932
TGGGAGT100.006447917148.507936
TAAAGAG100.006447917148.507933
CGGTAAA300.001680180274.253972
CCGGTAA350.00309656663.646261
GGGAGCT255.034375E-429.01085310-14
TCGCATT300.001446841824.21325135-39
CGCATTG300.001446841824.21325135-39
AGTTGCG300.001460027324.1757125-29
TCCGCGC300.001460027324.1757115-19
GTTGCGG300.001460027324.1757125-29
GCATTGC350.003543699621.215421
CGCAGTT350.003575831720.72203620-24
GCGCAGT350.003575831720.72203620-24
AATCCGC400.007465031518.5634926
AAATCCG400.007465031518.5634925
CCGCTAC400.00773876418.5634923
CGCTACC400.00773876418.5634924
ATAATAA400.00706935218.5634925
Produced by FastQC (version 0.11.5)