FastQCFastQC Report
Thu 2 Jun 2016
DRR028432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9058
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.5961580922941047No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA210.23183925811437403No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA190.20975932877014794No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA170.18767939942592185No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA170.18767939942592185No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA140.1545595054095827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120.1324795760653566No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA110.12143961139324355No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT110.12143961139324355No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA110.12143961139324355No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC100.11039964672113049No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC100.11039964672113049No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA100.11039964672113049No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACG100.006676677147.119327
AGAGTAG100.006907423145.46638
GAGTAGC100.006907423145.46639
GGGAATA100.006907423145.46639
GGTATCA203.528882E-4110.339491
TCAACGC353.0252972E-584.0681845
ATCAACG353.0252972E-584.0681844
ACGCAGA353.2017786E-583.12368
GTATCAA300.001767627373.559662
CGCAGAG406.207766E-572.733159
AACGCAG451.05127176E-465.386377
AGTACAT451.05127176E-465.386372
GAGTACA451.05127176E-465.386371
TATCAAC451.05127176E-465.386373
CAACGCA451.05127176E-465.386376
CATGGGA400.00552759255.1697466
ACATGGG604.357605E-449.0397723
GTACATG604.357605E-449.0397721
TACATGG604.357605E-449.0397722
CAGAGTA300.001444728124.5198867
Produced by FastQC (version 0.11.5)