FastQCFastQC Report
Thu 2 Jun 2016
DRR028431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223352
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20260.16561055199157726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17400.054.5350044
ATCAACG18050.052.5468863
CAACGCA18500.050.1016355
GTATCAA19500.047.1353031
GGTATCA12050.046.986661
GTACATG32950.045.301391
AACGCAG19800.044.9450846
TACATGG32300.043.0223162
ACATGGG34400.038.25863
TATCAAC25500.037.4832882
ACGCAGA22600.037.425677
CGCAGAG22200.035.9251068
CATGGGA18000.030.6495154
CATGGGG22750.028.453534
ATACCGT2353.7407517E-828.1667446
GCAGAGT25900.026.0361889
GAGTACA20750.025.160031
AATACCG2403.9010367E-521.455565
GTATAAT10400.020.5038571
GTATATA11500.019.821421
Produced by FastQC (version 0.11.5)