FastQCFastQC Report
Thu 2 Jun 2016
DRR028431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223352
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.12710977707152152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.11182390677417456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.076.297881
GTATCAA30750.062.936441
ATCAACG34500.053.9460223
TCAACGC34150.053.8627324
CAACGCA34350.053.549125
AACGCAG37800.049.044866
TATCAAC38850.048.2785382
ACGCAGA42600.042.3287167
CGCAGAG42700.041.55128
GTACATG30900.040.1119421
TACATGG32750.036.043062
ACATGGG31800.035.070263
GCAGAGT47750.034.7302139
CATGGGG20800.026.112194
CATGGGA17850.025.1535244
TATACTG11000.022.3836865
CAGAGTA45800.021.978339
GAGTACA22200.020.5694941
GTATAGA9750.020.072211
TCTATAC9150.018.2033823
Produced by FastQC (version 0.11.5)