FastQCFastQC Report
Thu 2 Jun 2016
DRR028430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705983
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10460.14816220787186093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.063.121121
GTACATG24250.049.195431
TCAACGC11100.046.4544
GTATCAA11850.045.9869771
TACATGG24500.045.387092
CAACGCA11800.043.073995
ATCAACG11800.043.0614433
AACGCAG12250.040.2772876
ACATGGG26200.039.069243
TATCAAC14500.037.074592
CATGGGG15600.032.581614
CATGGGA13250.030.5774
CGCAGAG15000.030.4721248
ACGCAGA14950.029.555047
ATACGGG2252.4179826E-829.4565267
CGACACG1050.007679607327.6391169
AATACGG2806.9321686E-926.3004676
GGACCGA1709.2699804E-525.991056
TACGGGC2559.4110874E-825.6068258
GCAGAGT16650.024.8378529
Produced by FastQC (version 0.11.5)