FastQCFastQC Report
Thu 2 Jun 2016
DRR028430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705983
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15490.2194103824029757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14920.21133653359925098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.1252154796928538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.079.035741
GTATCAA20300.058.1736831
AGTACGC450.00968894948.2688333
ATCAACG25400.045.89343
TCAACGC25150.045.7738274
CAACGCA26000.044.2773745
TATCAAC28050.042.3320242
AACGCAG27550.041.7862666
GTACATG24300.039.3552471
TACATGG24700.037.8138432
ACGCAGA31400.035.971047
ACATGGG25500.035.491793
CGCAGAG31800.035.2908948
GCAGAGT36350.029.4791769
CATGGGA13450.029.0689644
GTGGTAT5550.028.7186951
GCTATAC1855.8736186E-627.3958243
ACCGTAT1358.1436156E-426.816028
CAGAGTA35650.026.4023089
ATACCGT1400.001006982925.8583056
Produced by FastQC (version 0.11.5)