FastQCFastQC Report
Thu 2 Jun 2016
DRR028429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959108
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15790.16463213736096458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.057.2608681
GTATCAA17450.054.8873021
TCAACGC16600.054.614454
CAACGCA17800.050.932585
ATCAACG17800.050.9106983
AACGCAG18700.048.4708636
GTACATG33900.043.249131
TATCAAC21850.042.4834482
TACATGG35150.040.2417222
ACGCAGA22250.039.414647
ACATGGG36700.037.138953
CGCAGAG23250.036.5225038
GCAGAGT26050.029.8108049
CATGGGA19400.029.2549654
CATGGGG22850.028.3861854
GAGTACA25050.027.0585231
AGTACAT26350.024.324242
GTATAGG4051.5639671E-820.0106721
AGAGTAC22700.019.4821075
TAATACA12750.019.0771874
Produced by FastQC (version 0.11.5)