FastQCFastQC Report
Thu 2 Jun 2016
DRR028429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959108
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.1612957039248969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.1453433815586983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.081.093151
GTATCAA28700.061.865641
TCAACGC32450.053.337354
ATCAACG33050.052.3690453
CAACGCA33800.051.207015
AACGCAG35200.048.9646346
TATCAAC35950.048.7488822
ACGCAGA41050.041.457487
CGCAGAG42100.039.907468
GTACATG34200.038.1427231
TACATGG34550.035.6327322
ACATGGG34600.034.3254283
GCAGAGT47300.034.2953389
CCGTTCG850.00275713234.0791939
TAGATCG1504.4324806E-528.9673145
CATGGGG21450.026.6715354
ATGGGCG1958.830964E-625.9963075
GAGTACA26550.023.7476161
ATTATAC9600.023.385073
CATGGGA19400.023.1439864
Produced by FastQC (version 0.11.5)