FastQCFastQC Report
Thu 2 Jun 2016
DRR028427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198220
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18940.15806780057084677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG14300.053.4865233
TCAACGC14300.052.9790344
GGTATCA10650.049.724131
CAACGCA15450.047.609435
GTATCAA17800.044.6260031
AACGCAG16050.044.4433256
GTACATG27050.038.338591
TATCAAC20000.038.2428632
TACATGG27000.036.7719842
ACGCAGA19100.035.4228027
CGCAGAG18600.034.684038
ACATGGG31600.031.1863693
GCAGAGT22950.023.3723019
CATGGGG21200.022.8987064
GTATTAG9100.022.6308521
AGTACAT20450.021.5776942
GAGTACA21150.021.5608331
GCGTGCG1950.006849067818.5860829
ACTATGC5901.2732926E-1118.4285728
TCTGTCG4455.5539203E-817.9178168
Produced by FastQC (version 0.11.5)