FastQCFastQC Report
Thu 2 Jun 2016
DRR028427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198220
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.17601108310660812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.15823471482699336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.082.3205641
GTATCAA33600.063.3868371
TCAACGC38500.054.7320864
ATCAACG39350.054.101883
CAACGCA39150.053.638425
AACGCAG42000.050.343496
TATCAAC43500.048.9404372
ACGCAGA48150.043.4621627
CGCAGAG48350.042.9828458
GTACATG30100.039.2294431
GCAGAGT54500.037.468169
TACATGG30950.036.4983862
ACATGGG31150.033.7069663
TGGTATC9300.026.4731562
GTGGTAT9050.026.415351
CATGGGG21500.025.2599534
CAGAGTA54000.022.796339
CGTAGCC1600.00219083222.628713
GTATACG1700.003107514521.30651
CATGGGA17450.019.9184514
Produced by FastQC (version 0.11.5)