DRR028426_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	DRR028426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5265
Sequences flagged as poor quality	0
Sequence length	151
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66	1.2535612535612535	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61	1.1585944919278253	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52	0.9876543209876543	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	39	0.7407407407407408	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.41785375118708457	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.3798670465337132	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	16	0.30389363722697055	No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	13	0.24691358024691357	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	12	0.2279202279202279	No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG	11	0.20892687559354228	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	10	0.1899335232668566	No Hit
CATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAA	10	0.1899335232668566	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	9	0.17094017094017094	No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC	9	0.17094017094017094	No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA	9	0.17094017094017094	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	8	0.15194681861348527	TruSeq Adapter, Index 1 (95% over 21bp)
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG	8	0.15194681861348527	No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT	8	0.15194681861348527	No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC	8	0.15194681861348527	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	7	0.13295346628679963	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	7	0.13295346628679963	No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA	7	0.13295346628679963	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	7	0.13295346628679963	No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG	7	0.13295346628679963	No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT	7	0.13295346628679963	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.13295346628679963	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	7	0.13295346628679963	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	6	0.11396011396011395	No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT	6	0.11396011396011395	No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG	6	0.11396011396011395	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	6	0.11396011396011395	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.11396011396011395	No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC	6	0.11396011396011395	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATAAG	10	0.0069361036	144.69525	1
GGTATCA	25	8.9460437E-4	86.81715	1
TCAACGC	85	1.8189894E-12	76.60336	3
TATCAAC	85	1.8189894E-12	76.60336	1
ATCAACG	85	1.8189894E-12	76.60336	2
CAACGCA	100	7.2759576E-12	65.11286	4
AACGCAG	110	1.6370905E-11	59.19351	5
ACGCAGA	110	1.6370905E-11	59.19351	6
CGCAGAG	110	1.6370905E-11	59.19351	7
GCAGAGT	115	1.8044375E-9	50.328777	8
GTATCAA	45	0.009193761	48.231743	1
CAGAGTA	120	2.6302587E-9	48.231743	9
GAGTACT	85	1.2587407E-9	20.50568	10-14
AGTACTT	90	2.564775E-9	19.366474	10-14
AGAGTAC	125	1.8189894E-12	18.591816	10-14
ACTTTTT	90	5.165748E-8	17.85502	15-19
GTACTTT	90	3.283241E-4	16.077248	6
TACTTTT	90	3.283241E-4	16.077248	7
CTTTTTT	130	3.3313736E-6	12.361168	15-19
CGAGCCC	130	6.38371E-4	10.017363	135-139

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content