FastQCFastQC Report
Thu 2 Jun 2016
DRR028425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145964
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18600.1623087636260825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.049.6831051
TCAACGC13550.041.2661934
GTACATG29800.040.4809271
ATCAACG14550.038.923493
CAACGCA14750.037.4134945
TACATGG30450.036.713082
GTATCAA18200.035.565931
AACGCAG16000.034.0245976
ACATGGG34000.031.7996563
TATCAAC20600.030.7040582
ACGCAGA17500.030.2687957
GTATAGG4850.028.8160721
CGCAGAG18050.027.3233878
CAACGCG1100.00900732826.7515246
CATGGGG24750.024.078534
CGTACTG1750.003358521721.0228215
CATGGGA16750.019.7659554
GAGTACA20350.019.518761
GTATTAG10950.019.4807991
TATAGGG5508.0035534E-1118.7210372
Produced by FastQC (version 0.11.5)