FastQCFastQC Report
Thu 2 Jun 2016
DRR028425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145964
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.14782314278633535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.1286253320348632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.077.2425161
GTATCAA29050.058.6259571
ATCAACG32900.051.060043
TCAACGC33000.050.685894
CAACGCA34100.049.2632035
AACGCAG36150.046.4695786
TATCAAC38600.043.7076722
ACGCAGA39500.042.7117967
CGCAGAG39200.042.6692478
GCAGAGT47500.033.536539
GTACATG30250.031.384421
TACATGG31850.028.872432
ACATGGG31850.028.4177473
CAGAGTA43750.023.9982199
GAGTACA23000.023.9471571
TAGATCG1550.001821997923.3575535
CATGGGG22300.021.1055934
AGTACAT25600.020.930562
TATAGTG6600.020.8448475
CATGGGA16350.020.8146534
Produced by FastQC (version 0.11.5)