FastQCFastQC Report
Thu 2 Jun 2016
DRR028424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999994
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16070.16070096420578522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.058.4981841
TCAACGC15500.053.583614
GTATCAA16150.052.329161
ATCAACG15800.052.0930023
CAACGCA15950.050.2285925
AACGCAG17850.044.0495766
TATCAAC21550.039.216522
GTACATG25200.039.0771331
TACATGG24450.037.8712922
ACGCAGA19250.037.7920657
CGCAGAG18150.036.3333668
ACATGGG28700.028.6783773
AGTACCG1957.883837E-626.3790787
GCAGAGT21400.026.075939
GTACAGG7300.022.1471711
GTATACG2155.06985E-420.22449
GTATATA12200.019.8779951
GAGTACA18950.019.7778661
CATGGGA13100.019.637314
CATGGGG20100.019.0148334
Produced by FastQC (version 0.11.5)