FastQCFastQC Report
Thu 2 Jun 2016
DRR028424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999994
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.21430128580771485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.19510117060702364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.11070066420398522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.083.2135851
GTATCAA30400.059.6080741
TCAACGC35950.049.566984
ATCAACG36200.049.4247673
CAACGCA36850.048.356395
AACGCAG38500.045.9076776
TATCAAC40500.044.713762
ACGCAGA44500.039.3923237
CGCAGAG45000.038.310758
GTGGTAT6700.036.7826271
GTACATG25450.032.468011
TAGGTCG900.003643882232.1939135
GCAGAGT51650.032.1159979
TGGTATC7150.030.3928532
TACATGG26750.030.0576782
CCTAACG1000.006100600628.974518145
TATACCG1000.006100600628.9745186
ACATGGG30250.024.9037193
CAGAGTA50950.023.173939
CATGGGA14850.022.9259664
Produced by FastQC (version 0.11.5)