FastQCFastQC Report
Thu 2 Jun 2016
DRR028423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238727
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19630.15846913807481391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.052.7207261
GTATCAA19000.048.4454571
ATCAACG17300.048.096233
TCAACGC17250.047.8246544
CAACGCA17650.046.3253975
AACGCAG19100.042.0267566
GTACATG30150.038.8334961
TATCAAC23000.038.4177632
ACGCAGA20950.035.856427
TACATGG30850.035.563932
CGCAGAG21550.034.012358
ACATGGG35650.029.329693
ATAATAC12350.027.4264473
GCAGAGT26750.024.1460749
CATGGGG27200.023.0182824
GTATATA13200.020.6407031
GAGTACA22800.019.7011551
TCTATAC8100.019.090313
CTATACA7200.018.4146254
ACGCATA2000.0078725718.1427258
Produced by FastQC (version 0.11.5)