FastQCFastQC Report
Thu 2 Jun 2016
DRR028423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238727
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.1943931148671176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.18309118958414566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.1005871350184504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.083.128841
GTATCAA37200.060.1979331
ATCAACG43350.050.7787743
TCAACGC43550.050.379314
CAACGCA44550.049.2484635
AACGCAG46950.047.0394256
TATCAAC48550.045.638352
ACGCAGA54200.040.3464557
CGCAGAG54400.039.9319088
GTGGTAT8800.035.4121131
GCAGAGT61250.033.2198759
GTACATG32150.030.6565971
TGGTATC10050.029.5403422
TACATGG34000.029.3898872
ACATGGG35050.027.6830863
ACCGATA1100.00973128326.330868
TCTGTCG3803.6925485E-1022.8662748
GAGTACA24850.021.580991
CAGAGTA58000.021.3484259
CATGGGA16100.020.6885344
Produced by FastQC (version 0.11.5)