FastQCFastQC Report
Thu 2 Jun 2016
DRR028422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001479
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16160.1613613465684253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.055.5825271
GTATCAA16450.052.81581
TCAACGC15850.051.1067164
CAACGCA16850.048.073685
ATCAACG17500.046.2809643
AACGCAG17500.045.8578726
GTACATG32500.042.591431
ACGCAGA20050.039.291167
TACATGG34550.038.1463242
TATCAAC22650.037.0582052
CGCAGAG21650.034.8647088
ACATGGG37550.033.922193
GCAGAGT25500.027.6084569
GAGTACA23500.026.0050681
CATGGGG31350.025.8386444
AGTACAT25200.024.2507572
CATGGGA12850.021.776854
GTATATA6400.020.7081011
GTAATAC5102.7284841E-1120.2118283
CTACGCA1900.0053801619.3789834
Produced by FastQC (version 0.11.5)