FastQCFastQC Report
Thu 2 Jun 2016
DRR028422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001479
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.14099147361053002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.13310314045526667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.074.207821
GTATCAA24700.058.3824731
TCAACGC29450.048.9341624
ATCAACG30250.048.118833
CAACGCA30500.047.486985
AACGCAG31850.045.246826
TATCAAC34300.042.437162
GTACATG37150.041.1575931
ACGCAGA37600.038.3274237
TACATGG39850.037.0720182
CGCAGAG39650.036.3458068
ACATGGG41000.034.089283
GCAGAGT44600.030.5258249
CATGGGG30600.030.0556894
GAGTACA26850.025.3693851
CATGGGA13150.023.1295914
AGTACAT28700.022.7093662
CAGAGTA44050.022.6870259
GTATATA5353.6379788E-1220.31721
AGTGCGC1800.00433550420.1160138
Produced by FastQC (version 0.11.5)