FastQCFastQC Report
Thu 2 Jun 2016
DRR028421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128305
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17300.1533273361369488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16050.055.9196054
ATCAACG16450.054.9895743
CAACGCA16750.053.5851025
GTATCAA18150.051.4644931
GGTATCA10850.049.4850231
AACGCAG18600.048.2444346
TATCAAC21900.042.3143162
GTACATG30200.040.6714941
ACGCAGA20850.040.2160267
CGCAGAG21450.037.5253878
TACATGG31600.036.074932
ACATGGG35700.029.6643923
GCAGAGT24800.028.6565139
CATGGGG27200.022.4485594
GTATAAT10150.021.0141871
TAACGGT1850.004638443719.8828034
GTATAGA7000.018.9127671
GAGTACA22550.018.5912651
ATTAGAC5951.2732926E-1118.5402433
CATGGGA16450.017.4412654
Produced by FastQC (version 0.11.5)