FastQCFastQC Report
Thu 2 Jun 2016
DRR028421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128305
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.1716734393625837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.15252967947496465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.084.266751
GTATCAA31750.060.4713861
TCAACGC36050.052.6298374
ATCAACG36550.051.9098663
CAACGCA37450.050.6623655
AACGCAG39100.049.0800676
TATCAAC41900.044.7632642
ACGCAGA44950.042.5314527
GTACATG31550.042.4834861
CGCAGAG45450.041.904238
TACATGG34150.037.3214042
GCAGAGT50950.036.3857889
ACATGGG35400.034.5715983
CATGGGG24350.027.6579514
GACCGTG1701.0395408E-425.5586767
GTGGTAT7200.024.1505221
CATGGGA16800.022.84564
CAGAGTA48700.022.30489
CTATACA6700.020.5359524
CTAATAC8650.020.0923673
Produced by FastQC (version 0.11.5)