FastQCFastQC Report
Thu 2 Jun 2016
DRR028420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883096
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55650.2955239669140607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15100.052.304074
ATCAACG15900.050.583843
CAACGCA17900.044.9332125
GTATCAA21050.042.696721
AACGCAG18950.041.2952846
GTACATG36900.038.106611
CGCAGAG20800.036.583178
ACGCAGA21400.035.556537
TATCAAC24600.035.3509862
GGTATCA13200.034.5932661
TACATGG39400.033.291842
ACATGGG44100.029.4103623
CATGGGG36950.026.080934
CTAGACG1701.03584214E-425.5733874
GCAGAGT33250.021.5779699
GTCGTAC1700.003098467621.3179781
GTCTCGC4901.8189894E-1120.7088951
TATACCG1800.00432228520.127745
GTATAAG10300.018.9999071
GTATAGA14500.018.4951841
Produced by FastQC (version 0.11.5)