FastQCFastQC Report
Thu 2 Jun 2016
DRR028420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883096
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.19659114564525654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.16292318607229794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.080.0599751
GTATCAA48650.065.007111
ATCAACG55050.056.3474853
TCAACGC55150.055.9824834
CAACGCA57750.052.607325
AACGCAG58500.052.1807566
TATCAAC61450.050.7147942
ACGCAGA65050.046.7036557
CGCAGAG66400.045.6449138
GCAGAGT80400.036.9753239
AACGCCG1501.1336542E-633.836936
GTACATG39350.032.074641
TACATGG43450.029.8573552
ACATGGG42400.029.2292963
GTCGTAC1555.4840668E-528.0786881
CATGGGG37750.025.1502574
ACGCCGA2101.554736E-524.1692357
TATACCG1600.002174515322.6586575
GTATTAG14950.022.3189561
CAGAGTA77450.022.0940199
Produced by FastQC (version 0.11.5)