FastQCFastQC Report
Thu 2 Jun 2016
DRR028419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788328
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23910.30330014917648496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9740.12355263291421845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9750.050.5384251
TCAACGC8350.048.585694
ATCAACG8400.048.2903333
CGCAGAG9000.044.2858778
GGTATCA5150.042.2115671
AACGCAG9900.040.9814456
ACGCAGA10050.040.3749247
GTACATG14900.040.36541
TACCGAG907.506213E-540.2547657
TATCAAC11600.039.9695052
GTACCGA1105.2881805E-639.5178226
CAACGCA10550.039.1407555
TACATGG14150.036.350362
TGTACCG1209.611358E-636.2223635
CGTATCC1051.8561126E-434.515068145
ACCGAGT1102.442034E-432.939918
GCAGAGT12100.032.3451239
GTCTAAG2051.1435986E-831.8131071
GTATTAA11400.024.7900371
CATGGGG12650.024.625484
Produced by FastQC (version 0.11.5)