FastQCFastQC Report
Thu 2 Jun 2016
DRR028419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788328
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.43053145391258457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28280.358733928009661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.19928253214398067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.0103.838921
GTATCAA33000.074.7399141
ATCAACG42500.057.4702033
TCAACGC42650.057.268084
TATCAAC43400.055.944432
AACGCAG44350.055.104376
CAACGCA44200.054.7991685
CGCAGAG49050.049.38078
ACGCAGA49300.049.130297
TTAGCGC657.32634E-444.6268658
GCAGAGT54250.043.4443559
TAGCGCA750.001481320538.6766179
TGGTATC6050.037.1371082
GTGGTAT6850.036.006091
GTACATG15350.031.6631431
TACATGG13400.030.829882
TCGACCT950.004720747530.5341729
ACGCCGA1000.00606605629.0074627
CTTAGCG1050.00769780327.6261567
ACATGGG14000.026.9201243
Produced by FastQC (version 0.11.5)