FastQCFastQC Report
Thu 2 Jun 2016
DRR028418_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028418_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38867
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA404410.404713510175727No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3710.9545372681194844No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.7306969923070986No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.5377312372964211No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.4759821956930044No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.3216095916844624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1120.2881621941492783No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.23155890601281293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.18782000154372605No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.18010137134329895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.16723698767592043No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.16466411094244474No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.15951835747549337No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.15951835747549337No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA560.14408109707463915No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.12349808320683356No Hit
ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.11063369953945507No Hit
AAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.11063369953945507No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.10548794607250365No Hit
GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10291506933902796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTTA100.0070789144.903351
ACATAAG203.8479973E-4108.677515
TACATAG352.7224087E-7103.502392
TACATGA451.0690201E-896.6022342
GTACATA554.0381565E-1092.211221
ACATGAG406.0305683E-790.56463
CATGAGG259.3431177E-486.942014
ACATAGG353.4069693E-582.801913
GGTATCA1350.080.501861
CATGGAA554.303547E-879.038194
GTACAAA300.001926790372.4516751
ATGGAAA502.2736604E-672.4516755
ACATAGA502.2736604E-672.4516753
GAACATA851.6370905E-1068.189811
GTACATG4400.067.511791
ACATGGA1009.094947E-1265.2065053
GGGGCCC350.003550092762.1014338
TACATGG4100.061.848992
GTATCAA2100.055.201281
CATAGAA553.191019E-452.6921274
Produced by FastQC (version 0.11.5)