FastQCFastQC Report
Thu 2 Jun 2016
DRR028417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239547
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28731.1993471009864454No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7140.29806259314456035No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG4630.19328148547049223No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC3900.16280729877644054No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT3860.1611374803274514No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC3490.14569165967430192No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.12356656522519589No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2850.11897456449047578No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA2840.1185571098782285No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT2820.11772220065373391No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT2720.11354765453126109No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC2550.10645092612305727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGA1500.096.61223
TACATGA1000.094.196892
CATGGAA1000.094.177164
GGTATCA2650.092.985941
GCTCTAG406.115988E-790.592911
GTATCAA6850.068.7712551
GATGACC1300.061.3372849
ATAGATG502.0106454E-457.979476
TGTGGAC651.0947588E-555.73782
ATCAACG7250.053.969573
GTGGACC1101.6752892E-952.6975633
TCAACGC7350.052.238514
GGACCAG1258.913048E-1152.170585
GTACATG16100.051.3172261
CAACGCA7500.050.2383425
TACATGG14600.049.629552
GATTTGC450.00964369448.3162237
ACCAGGT1203.612513E-948.3162237
TTTCAAC450.00965168248.30612
GTACGGA1255.18412E-946.383576
Produced by FastQC (version 0.11.5)