Basic Statistics
Measure | Value |
---|---|
Filename | DRR028417_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 239547 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2873 | 1.1993471009864454 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 714 | 0.29806259314456035 | No Hit |
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG | 463 | 0.19328148547049223 | No Hit |
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC | 390 | 0.16280729877644054 | No Hit |
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT | 386 | 0.1611374803274514 | No Hit |
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC | 349 | 0.14569165967430192 | No Hit |
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 296 | 0.12356656522519589 | No Hit |
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG | 285 | 0.11897456449047578 | No Hit |
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA | 284 | 0.1185571098782285 | No Hit |
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT | 282 | 0.11772220065373391 | No Hit |
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT | 272 | 0.11354765453126109 | No Hit |
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC | 255 | 0.10645092612305727 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACATGGA | 150 | 0.0 | 96.6122 | 3 |
TACATGA | 100 | 0.0 | 94.19689 | 2 |
CATGGAA | 100 | 0.0 | 94.17716 | 4 |
GGTATCA | 265 | 0.0 | 92.98594 | 1 |
GCTCTAG | 40 | 6.115988E-7 | 90.59291 | 1 |
GTATCAA | 685 | 0.0 | 68.771255 | 1 |
GATGACC | 130 | 0.0 | 61.337284 | 9 |
ATAGATG | 50 | 2.0106454E-4 | 57.97947 | 6 |
TGTGGAC | 65 | 1.0947588E-5 | 55.7378 | 2 |
ATCAACG | 725 | 0.0 | 53.96957 | 3 |
GTGGACC | 110 | 1.6752892E-9 | 52.697563 | 3 |
TCAACGC | 735 | 0.0 | 52.23851 | 4 |
GGACCAG | 125 | 8.913048E-11 | 52.17058 | 5 |
GTACATG | 1610 | 0.0 | 51.317226 | 1 |
CAACGCA | 750 | 0.0 | 50.238342 | 5 |
TACATGG | 1460 | 0.0 | 49.62955 | 2 |
GATTTGC | 45 | 0.009643694 | 48.316223 | 7 |
ACCAGGT | 120 | 3.612513E-9 | 48.316223 | 7 |
TTTCAAC | 45 | 0.009651682 | 48.3061 | 2 |
GTACGGA | 125 | 5.18412E-9 | 46.38357 | 6 |