FastQCFastQC Report
Thu 2 Jun 2016
DRR028417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239547
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33891.4147536809060435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28851.2043565563334127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.7368073906164553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.28512150016489457No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG5460.22793021828701673No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT4740.19787348620521233No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC4580.1911942124092558No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.18201021093981556No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.18034039249082645No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC3740.156128024980484No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG3390.14151711355182908No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT3370.1406822043273345No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG3300.13776002204160354No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA3170.13233311208238885No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.1306632936333997No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC3080.12857602057216327No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT3030.12648874751092687No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT2970.12398401983744317No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA2890.1206443829394649No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.11146038147002467No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT2580.10770328995979912No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.10770328995979912No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC2570.10728583534755183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2560.10686838073530455No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC2540.10603347151081No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT2510.10478110767406813No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT2410.10060656155159531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG202.1238648E-6145.046596
GGTATCA8900.0122.204751
GTGGTAT1200.0102.719871
CGCCGAG353.4264624E-582.8837668
TGGTATC1500.082.141592
GTATCAA21600.074.522261
CAACGCC406.642267E-572.523295
GTACGGA651.3904173E-766.944586
AATGCGT350.00355865962.1628236
CACTCTG350.00355865962.1628235
AGAGGAC350.00355865962.1628235
AGTACGG702.3218126E-762.1628235
GTCTCAC350.00356160662.149841
GCTCTAG350.00356160662.149841
GTTATCA502.0060291E-458.006521
GGATGAC902.035631E-856.4070058
ATCAACG28650.056.1608623
TCAACGC29350.054.574484
AAATGCG400.006037609754.3924675
GTCTCGC400.006042602554.3811071
Produced by FastQC (version 0.11.5)