FastQCFastQC Report
Thu 2 Jun 2016
DRR028416_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028416_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90718
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.35384377962477126No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2330.25683987742234177No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1270.13999426795123351No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT1140.1256641460349655No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.11133402411869751No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1000.11023170704821536No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC990.1091293899777332No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG970.10692475583676889No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA960.10582243876628673No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA920.10141317048435812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT100.0070905336144.929669
GGTATCA1150.0107.121911
TACATGA301.5953892E-596.619762
TACTAGA451.2273231E-680.5164641
GCAGAGG607.975723E-872.464829
TAACCGT300.00193799872.424697
TATAGTG502.3034463E-672.424695
GTATCAA2350.064.755791
GAAGTGT451.1941333E-464.377516
AACCGTG350.003570678262.0783128
GAATAAG350.003570678262.0783126
ATACCTT606.797507E-660.353916
CTATAGT502.0107244E-457.9397544
ATCAACG2850.055.906783
TGGGAAC400.00605799954.3185278
TCAACGC2950.054.0116354
CAACGCA3150.050.5823255
GTCTAAT604.9328385E-448.309881
TCTAATC450.0096294148.309882
ACCGTGC450.0096294148.309889