FastQCFastQC Report
Thu 2 Jun 2016
DRR028416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90718
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.8730351198218655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.8311470711435438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.4662801208139509No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT2190.2414074384355916No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1800.19841707268678763No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.17637073127714456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.16755219471328733No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1470.16204060936087658No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1290.1421989020921978No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1240.13668731673978704No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1170.12897109724641195No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1130.12456182896448335No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG1120.12345951189400119No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT1010.11133402411869751No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC990.1091293899777332No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT980.10802707290725104No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC970.10692475583676889No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC970.10692475583676889No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA920.10141317048435812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG151.2245837E-4145.05241
TCCAAGT100.007072661145.05241
CATAGCG100.0070843366144.972442
ACAATTG100.0070843366144.972449
GGTATCA2600.0108.789311
GTGGTAT553.6379788E-12105.492661
TGGTATC702.910383E-1182.841392
GATGACC300.001931508872.486229
GTATCAA5250.064.9282151
TGTCTGC350.003550966762.1653141
ATGGGAC350.003558738462.1310427
GGATGAC350.003558738462.1310428
CTACGTC400.00603777154.3646623
CTTAGGG400.00603777154.3646623
CTAGAGG858.845982E-751.1667444
CATGGGA2000.050.7403534
TACATGG3300.050.5206952
GTACATG3450.050.4530071
CCCTCAA450.0096183448.3241468
ACAGCCG450.0096183448.3241468
Produced by FastQC (version 0.11.5)