FastQCFastQC Report
Thu 2 Jun 2016
DRR028415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987571
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58540.29453035891548024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19950.049.7600334
ATCAACG20400.048.3071823
CAACGCA22400.043.995155
AACGCAG23650.041.976226
GGTATCA15950.041.354831
GTATCAA27450.039.0809331
GTACATG44750.038.064521
TATCAAC28500.035.6028562
TACATGG47050.034.813722
ACGCAGA27700.034.533537
CGCAGAG28850.032.6570748
ACATGGG53600.028.6598573
CGTGTCG2259.142077E-725.769035145
CATGGGG47250.024.2302684
CGCGACA1902.1992691E-422.886974145
GCAGAGT39200.022.7415859
GTATAAG12850.022.563291
GTATAGG9300.021.823311
GAGTACA33650.021.7561421
GTTAGAC5850.019.818333
Produced by FastQC (version 0.11.5)