FastQCFastQC Report
Thu 2 Jun 2016
DRR028415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987571
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37450.18842094194370917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30270.1522964462653158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.067.287031
GTATCAA52000.064.31291
ATCAACG57100.057.6256523
TCAACGC57600.057.1254274
CAACGCA59800.054.5527575
AACGCAG62200.052.7974786
TATCAAC64750.050.9305842
ACGCAGA69700.047.0122457
CGCAGAG71550.045.6953778
GCAGAGT85950.037.0274669
GTACATG45400.031.3184871
TACATGG48000.029.4442422
ACATGGG48800.027.3271353
GTATACG2051.2850645E-524.7710691
CATGGGG43700.023.7165074
CAGAGTA84700.023.0236539
GTGGTAT16100.020.726811
TGGTATC15900.019.6010062
GTATAGA14100.019.5508211
AGTACCG2659.3609924E-519.1495045
Produced by FastQC (version 0.11.5)