FastQCFastQC Report
Thu 2 Jun 2016
DRR028414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833243
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52320.28539588041519864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24200.051.5170361
TCAACGC22900.049.677444
ATCAACG23350.048.408423
GGTATCA17200.046.7769971
CAACGCA24900.045.106525
AACGCAG26250.043.0627986
GTACATG55200.041.6253551
TATCAAC29650.040.3253442
TACATGG54050.039.4158132
ACGCAGA30050.036.8938457
ACATGGG57650.035.820263
CGCAGAG31800.033.0405968
CATGGGG48200.030.5171554
GAGTACA37000.023.7039871
GCAGAGT42750.022.5460289
ACCGCGT2353.7266014E-521.5842598
AGTACAT41850.020.604892
GTGTACG2505.9885875E-520.2953151
ATGGGGG34850.019.9606845
TATACTG15500.017.2972475
Produced by FastQC (version 0.11.5)