FastQCFastQC Report
Thu 2 Jun 2016
DRR028414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833243
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.22855671615819614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33710.18388178762989957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18720.10211412235039216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.074.956181
GTATCAA51500.064.780381
ATCAACG60550.053.9847263
TCAACGC61000.053.705294
CAACGCA64600.050.7234955
TATCAAC66400.049.8834652
AACGCAG66300.049.641586
ACGCAGA77600.042.3194247
CGCAGAG79500.041.4903958
GTACATG54700.038.7158131
TACATGG55550.036.793712
GCAGAGT90400.035.5253649
ACATGGG55700.035.5235183
CATGGGG49150.031.5572224
GTGGTAT14600.027.3213841
ATGGGGG33800.024.4507085
GAGTACA36050.023.1358491
CAGAGTA87100.022.305919
TGGTATC16750.021.2026332
TATACTG14100.019.0233025
Produced by FastQC (version 0.11.5)