FastQCFastQC Report
Thu 2 Jun 2016
DRR028413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837836
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55140.3000267706150059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17250.058.386844
ATCAACG17750.057.558573
CAACGCA19850.051.1070145
AACGCAG21050.047.8493086
GGTATCA14200.042.8756451
GTATCAA24050.042.7949941
TATCAAC24700.040.78182
ACGCAGA24200.039.227687
CGCAGAG23900.038.507258
GTACATG39200.036.7948231
TACATGG42750.031.699622
ACATGGG49300.026.1614823
GCAGAGT34850.024.3294149
CATGGGG41300.022.8077564
GTCGTAC1700.00309865621.3177221
ACTGCGT3001.1107799E-519.3243738
TACACTG18650.017.8727765
GTATTAG14350.017.1730211
GTCTAGC5501.9408617E-917.1316971
ATGGGGG31750.017.1171115
Produced by FastQC (version 0.11.5)