FastQCFastQC Report
Thu 2 Jun 2016
DRR028413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837836
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37950.2064928535516771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30270.16470457646928235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.081.178351
GTATCAA48650.062.469331
ATCAACG54400.055.4211433
TCAACGC55200.054.749234
CAACGCA57900.052.087995
AACGCAG58800.051.414026
TATCAAC61150.049.0664832
ACGCAGA66750.045.0733347
CGCAGAG67600.044.7210738
GCAGAGT82550.035.480299
GTACATG40550.031.6605221
TACATGG42850.027.9070642
ACATGGG43350.027.2508163
GACCGAC1100.00967529626.3627197
GTCGTAC1701.02856706E-425.5998821
CATGGGG36350.025.1215934
CTATGCG1751.2565829E-424.8562769
ATGGGGG26950.024.2106575
CAGAGTA80750.023.253069
TGGTATC12800.020.3832572
Produced by FastQC (version 0.11.5)