FastQCFastQC Report
Thu 2 Jun 2016
DRR028412_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028412_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803260
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53670.2976276299590741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19700.052.224414
ATCAACG20850.049.6928063
CAACGCA21650.046.516645
AACGCAG22500.044.7593466
GTATCAA25200.044.292361
GGTATCA14850.043.9263081
GTACATG45450.039.8671071
ACGCAGA27400.035.963677
TATCAAC30800.035.7615932
CGCAGAG27700.035.575168
TACATGG45250.035.231252
ACATGGG52300.027.984223
CATGGGG42400.025.2899134
GCAGAGT37750.024.9552829
TAGACCG1550.001816004423.3716725
GAGTACA33350.023.2539421
GTACCGG1850.005057113719.5887581
TACCGTC2701.08479355E-418.7849457
AGTACAT38200.018.2109182
GTATAGT11100.017.6298831
Produced by FastQC (version 0.11.5)