FastQCFastQC Report
Thu 2 Jun 2016
DRR028412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803260
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45880.25442809134567396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37320.2069585084790879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.12000488005057507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.083.674771
GTATCAA54800.071.336161
TCAACGC64100.061.0553974
ATCAACG64450.060.723833
CAACGCA65750.058.7597545
AACGCAG68250.056.7135936
TATCAAC70700.054.9456982
ACGCAGA78500.049.308317
CGCAGAG79500.048.779268
GCAGAGT91650.040.651759
GTACATG44950.035.9812851
TACATGG46700.032.9008642
ACATGGG48450.031.2637333
CATGGGG41100.026.2743994
TAGTGCG1450.001228299524.994855
AACGCCG1751.2580307E-424.8520246
CTAGCGT1500.001497740924.1583394
TGGTATC14250.023.3954452
GTGGTAT15050.023.1316321
GAGTACA33050.023.0419561
Produced by FastQC (version 0.11.5)