FastQCFastQC Report
Thu 2 Jun 2016
DRR028411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911518
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54360.28438131369937397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16750.049.75014
ATCAACG17100.048.7318233
CAACGCA19100.043.6313065
GTATCAA23350.043.148371
AACGCAG20800.040.065296
GTACATG37450.036.193141
ACGCAGA22400.035.5859387
GGTATCA15350.035.4151731
CGCAGAG22450.034.8611078
TATCAAC27150.033.8983882
TACATGG40350.030.1724742
ACATGGG45350.025.086113
CATGGGG39950.022.3099824
GTATTAG14100.022.104761
GAGTACA30050.021.9499421
GTATAGA15150.021.5296171
GCAGAGT33800.021.2257799
GTATACG3457.5318894E-821.009581
TAGTACG3401.5238984E-619.1811454
GTCCTAT9350.018.6052741
Produced by FastQC (version 0.11.5)