FastQCFastQC Report
Thu 2 Jun 2016
DRR028411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911518
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35580.18613478920941368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.16123311420556857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.074.9737851
GTATCAA51850.063.7863461
TCAACGC58200.056.2841534
ATCAACG58500.055.9955183
CAACGCA60450.053.9663735
AACGCAG62000.052.7341426
TATCAAC65550.049.9731182
ACGCAGA69950.046.4298557
CGCAGAG70700.045.834788
GCAGAGT85100.037.4826359
GTACATG41550.028.9766851
TACATGG43050.027.2717232
CCGATCG1100.00967711126.3617559
CAGAGTA81750.025.7168229
TATACGG1500.001495390824.1649445
ACATGGG45000.024.1573583
GTGGTAT15450.021.1249491
TCTGTCG6200.019.8776158
AGAGTAC76350.019.32188210-14
GAGTACA34300.019.2423861
Produced by FastQC (version 0.11.5)