FastQCFastQC Report
Thu 2 Jun 2016
DRR028410_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028410_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929213
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56580.29328021322684433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19100.054.624834
ATCAACG20750.050.2824753
CAACGCA22400.047.2267885
GTATCAA24900.045.996221
AACGCAG23800.045.364436
GGTATCA14900.042.8115351
CGCAGAG27100.038.5074358
ACGCAGA28800.036.987387
GTACATG42400.036.756741
TATCAAC30900.035.418192
TACATGG44100.032.5412672
GCAGAGT35000.028.160799
ACATGGG48650.026.3610483
CATGGGG41550.024.2384414
GTATTAG14050.020.637071
GTCTAGC4652.055458E-1020.2653831
GAGTACA33850.019.4870951
GTATAGA14850.017.5727791
ACCGCGT2550.00159630117.0514938
ATTATAC20000.017.0270773
Produced by FastQC (version 0.11.5)