FastQCFastQC Report
Thu 2 Jun 2016
DRR028410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929213
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38590.20002975306510998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.15876940493351432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.084.715441
GTATCAA54650.062.0991131
ATCAACG61450.055.1943633
TCAACGC61400.055.121284
CAACGCA63450.052.5653575
AACGCAG65300.051.0761386
TATCAAC68650.049.3000222
ACGCAGA74400.044.731477
CGCAGAG74850.044.2688068
GCAGAGT89200.036.0903859
GTACATG42450.032.627621
TACATGG45400.029.2123182
ACATGGG47750.027.6228683
GAGTACA31250.023.2048991
CATGGGG41350.023.1349834
CAGAGTA87350.023.0757249
GTATAAG10400.023.0096681
AACGCCG2104.3137604E-420.7159736
GTGGTAT12600.019.5676231
AGAGTAC78950.019.09264810-14
Produced by FastQC (version 0.11.5)